How Big is a Bug ?
This experiment illustrates the differences in size between bacterial cells and virus particles - a classic microbiology experiment.
You have been given the following reagents:
- nitrocellulose filter, pore size 0.2 µm (200nm)
- mixed suspension of bacteriophage l (lambda) and Staphylococcus
(S. epidermidis is not susceptible to infection by l phage)
- 5ml syringe
- disposable spreader
- nutrient agar plates
- sterile tubes
- phage dilution buffer
Perform the experiment
© AJC 2007.