http://www.microbiologybytes.com/blog/2012/08/27/self-amplifying-rna-vaccines/ The latest news about microbiology Sat, 25 May 2013 09:27:33 +0000 hourly 1 http://wordpress.org/?v=3.5.1 http://www.microbiologybytes.com/blog/2012/08/27/self-amplifying-rna-vaccines/#comment-24795 AJ Cann Tue, 28 Aug 2012 07:16:59 +0000 http://www.microbiologybytes.com/blog/?p=5198#comment-24795 Surely the fact that it’s not just a transcription system to make particles is new?

]]> http://www.microbiologybytes.com/blog/2012/08/27/self-amplifying-rna-vaccines/#comment-24761 Ed Rybicki Mon, 27 Aug 2012 17:06:40 +0000 http://www.microbiologybytes.com/blog/?p=5198#comment-24761 …and having seen the paper, I note:
“The RNA was produced in vitro by an enzymatic transcription reaction from a linear pDNA template using a T7 RNA polymerase, thereby avoiding safety concerns and complex manufacturing issues associated with cell culture production of live viral vaccines, recombinant subunit proteins, and viral vectors.”
Yes: and making the delivery SOOOOOOO incredibly expensive as to be totally impractical for any purposes other than a proof of concept – and it really isn’t anything newer than the old Alphavax VEE vector, dressed up with in vitro-synthesised RNA and liposomes.
So thanks for the paper, Alan, but as far as a vaccine goes – FAIL!!!

]]> http://www.microbiologybytes.com/blog/2012/08/27/self-amplifying-rna-vaccines/#comment-24760 Ed Rybicki Mon, 27 Aug 2012 17:02:30 +0000 http://www.microbiologybytes.com/blog/?p=5198#comment-24760 …so: liposomes to deliver what amounts to a viral RNA that can’t make particles? OK…this is new, how??

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