Cryo-electron tomography of bacterial viruses

Bacteriophage Bacteriophage particles contain both simple and complex macromolecular assemblages and machines that enable them to regulate the infection process under diverse environmental conditions with a broad range of bacterial hosts. Recent developments in cryo-electron tomography (cryo-ET) make it possible to observe the interactions of bacteriophages with their host cells under native-state conditions at unprecedented resolution and in three-dimensions.

This review describes the application of cryo-ET to studies of bacteriophage attachment, genome ejection, assembly and egress. Current topics of investigation and future directions in the field are also discussed.

 

Cryo-electron tomography of bacterial viruses. (2013) Virology. 435(1): 179-186. doi: 10.1016/j.virol.2012.08.022