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Acanthamoeba polyphaga

Acanthamoeba species such as Acanthamoeba polyphaga (and their close relative Naegleria fowleri) are pathogenic free-living amoebae, i.e. opportunistic rather than obligate pathogens. These organisms are ubiquitous in the environment, in soil, water and air. Acanthamoeba is characterised by a feeding and dividing trophozoite that, in response to adverse conditions, can form a dormant cyst stage:


You can get a longer, better quality version of this video on the Microbiology Video Library CD.

Acanthamoeba is pathogenic to humans causing a rare but fatal encephalitis (infection of the brain) in the immunocompromised host and, more frequently, a potentially blinding infection of the cornea (keratitis). Prior to 1980's, amoebae had been reported from eye (ocular) infections only rarely - these cases were associated with trauma to the eye. In mid 1980's cases began to occur in wearers of contact lenses. Contact lens wearers are most at risk from acanthamoeba keratitis and account for 95% of reported cases. Poor hygiene practices such as failing to clean and disinfect lenses and rinsing them in tap water are known risk factors.

Acanthamoeba cysts

Non-motile cysts formed by Acanthamoeba (seen at the beginning of the video) are resistant to some disinfectants but can be killed by heat (e.g. 65°C for 30 minutes).

Ocular amoebic keratitis may be diagnosed by culturing corneal scrapings on nonnutrient agar overlaid with viable Escherichia coli.


You can get a longer, better quality version of this video on the Microbiology Video Library CD.

 

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Fearsome Fauna is an amusing and informative look into the secret world of the parasites that inhabits each of us.
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CoverMedical Microbiology Made Memorable
by S.H.Myint, S.Kilvington, A.Maggs, R.A.Swann
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The book provides a core text of medical microbiology for medical students and biological science courses. Each topic is presented as a double-page spread, roughly covering the content that might be found in one lecture. The left-hand page contains the descriptive text and the right-hand page has diagrams, flow charts etc.
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MicrobiologyBytes, 2007.